A police spokeswoman said a resident reported the possible sighting of 31-year-old Eric Frein early Tuesday afternoon near Route 390 in Barrett Township in Monroe County.
Trooper Connie Devens declined further comment on the report.
Frein is accused of having opened fire outside the Blooming Grove state police barracks on Sept. 12, killing Trooper Bryon Dickson and seriously wounding Trooper Alex Douglass.
Last week, a pair of possible sightings over a four-day period prompted closure of nearby schools.
Tuesday marked the 46th day of the manhunt.